The antibody may cross-react with c-myc protein by Flow Cytometry and Immunofluorescence assays. Phone: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: [email protected] Learn about our antibody labeling kits and their advantages. Product Description This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [Alexa Fluor ... BP Fluor 647 acid, Alexa Fluor 647 acid equivalent ... Zip Alexa Fluor Rapid Antibody Labeling Kits allow you to efficiently label your precious antibody with fluorescent dyes to create an antibody conjugate that is ready to use within 15 minutes. lysines). ASP7266, a novel antibody against human thymic stromal lymphopoietin receptor for the treatment of allergic diseases. Gold (9) . Alexa Fluor ™ 647 Tyramide SuperBoost Kit - Goat anti-Rabbit IgG (Cat. The dye has 4 sulfonate groups make it high water soluble and less aggregation in the aqueous solution. Alexa Fluor 647 maleimide is a thiol reactive bright green-fluorescent dye, it can be labeled to thiol containing biomolecules, such as primary antibody, secondary antibody, peptide, oligo, etc. Important Features of Microscale Protein Labeling Kits: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)) Cross-Reactivity. EdU | 5 EdU detection Incorporated EdU can be detected with a Click-iT® EdU Kit (Table 3) or with an available dye- or hapten-containing azide (Table 4). Alexa Fluor ® Antibody Labeling Kits. No. Quick Protocol. The dye is water soluble and pH-insensitive from pH 4 to pH 10. The unconjugated antibody #3670 reacts with human, mouse and rat GFAP protein. Alexa Fluor® 647 - conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. no. CST expects that GFAP (GA5) Mouse mAb (Alexa Fluor ® 647 Conjugate) will also recognize GFAP in these species. Zenon® Mouse IgG Labeling Kits | 4 Table 2 Zenon® Mouse IgG Labeling Kits available from Life Technologies Label Ex/Em* Catalog Number Mouse IgG 1 Mouse IgG 2a Mouse IgG 2b Alexa Fluor® dyes Alexa Fluor® 350 346/442 Z25000 Z25100 Z25200 Alexa Fluor® 405 402/421 Z25013 Z25113 Z25213 Alexa Fluor® 488 495/519 Z25002 Z25102 Z25202 Alexa Fluor® 532 531/554 Z25003 . It takes only 30 seconds hands-on time and conjugates are ready to use in less than 20 minutes without loss of antibodies. XCR1 antibody (Chemokine (C Motif) Receptor 1) (Alexa Fluor 647) Primary Antibody. View Mouse Monoclonal anti-Fc gamma RIIIB/CD16b Antibody (24) [Alexa Fluor® 647] (NBP3-12795AF647). This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. Alexa Fluor 647 is used for protein and antibody labeling, or nucleic acid applications with high labeling density. 332-0015 is the same as the 1 mg size. The Protein Labeling Kits provide a convenient means to covalently label proteins with the superior Alexa Fluor™, Pacific Blue™, Fluorescein-EX, and Oregon Green ™ 488 fluorescent dyes. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Molecular Probes® Alexa Fluor® Antibody Labeling Kits provide a convenient means to label small amounts of antibodies with Alexa Fluor® dyes (choice of 10 colors). Product Details anti-KDM2A Antibody (hide) AA 741-840. Alexa Fluor® 647** ~1300 650 665 239,000 0.03 0.00 . Excitation (nm) Emission (nm) Application Alexa Fluor® 488 A10468 496 519 Fluorescent label for use in imaging or flow . Heidelberg, Germany and Cambridge, UK, 14 August 2018 - Expedeon AG (Frankfurt: EXN; ISIN: DE000A1RFM03; Prime Standard) today announced the introduction of five new Alexa Fluor® labels to its range of Lightning-Link® antibody labelling kits. BP Fluor 647 is water soluble and pH-insensitive from pH 4 to pH 10. It can also be used to conjugate other proteins or peptides. SAIVI™ Alexa Fluor® 647 Antibody/Protein 1 mg-Labeling Kit | 3 1.3 Prepare ~500 µL of a ~2 mg/mL protein solution by diluting it with an appropriate buffer (see Protein Preparation) and add sodium bicarbonates as directed here, depending on the nature of the starting protein sample. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. BP Fluor 647 is spectrally similar to Cy5 . Alexa Fluor® 647 Conjugation Kit - Lightning-Link® (ab269823) provides a simple and rapid procedure to covalent labeling of antibodies with Alexa Fluor® 647 by targeting primary amine groups (e.g. The Antibody - Streptavidin Alexa Fluor ® 647 complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes. 332-0005 is the same as the 100 µg size.. Formalin-fixed, 300 micron-thick mouse kidney section was blocked, permeabilized and stained overnight with CD31 (clone MEC13.3) Alexa Fluor® 647 (red) at 5 µg/mL, Tubulin Beta 3 (TUBB3) (clone AA10) Alexa Fluor® 488 (green) at 2.5 µg/mL, and Podoplanin (clone Pmab-1) Alexa Fluor® 594 (blue) at 5 . Heikki Lanckriet, PhD, Expedeon. Detection of LC3B in HeLa Human Cell Line by Flow Cytometry. The label, when attached to the antibody, is located far away from its antigen binding domain. No. In The Journal of Pharmacology and Experimental Therapeutics on 2 November 2021 by Numazaki, M., Abe, M., et al.. B40912). Dye Antibody Labeling Kit. No. PRODUCT INFORMATION SHEET Version: 1 Revision Date: 08/08/2020 BP Fluor 647 /Cy5/ Cyanine 650 Antibody Labeling Kit Component The BP Fluor 647, Cy5, and Cyanine 650 conjugation kits are available in two formats, NHS and maleimide. Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab198324) ab198324 staining M6PR (cation independent) in CaCo2 cells. The conjugates are widely used in flow cytometry, immunohistochemistry, immunocytochemistry and western blotting. Dye Antibody Labeling Kit is for labeling antibodies with NHS ester or maleimide with popular dyes, such as fluorescein, Alexa Fluor 488, Cy3, Cy5, Cyanine 650, Alexa Fluor 647, and 800CW. For example, AE-1/AE-3 is a commonly used specific pan cytokeratin that detects cytokeratins 1-8, 10, 14-16 and 19 (1,3,6). This CAPG antibody is conjugated to Alexa Fluor 647. Validated Applications: Flow, ICC/IF, IHC, IHC-P. Validated Species: Human, Mouse, Guinea Pig.Range of directly conjugated antibodies available. This kit includes enough primary antibody to perform at least 20 IF-IC tests per antibody. Readilink(TM) protein labeling technology is the most robust and convenient tool for preparing fluorescent antibody conjugates used for fluorescence… 13-8400) and then detected with Alexa Fluor ™ 488 Tyramide SuperBoost Kit - Goat anti-Mouse IgG (Cat. ized with an Alexa Fluor 647 goat anti-chicken IgG (pseudocolored purple), goblet cells were labeled with an Alexa Fluor 594 conjugate of wheat germ agglutinin (red), and nuclei were labeled with anti-cdc6 peptide antibody and visualized with an Alexa Fluor 405 goat anti-mouse IgG (blue). The addition further expands Expedeon's range of labels, offering customers increased choice and flexibility for common fluorescence detection . View Rabbit Polyclonal anti-PIEZO1 Antibody [Alexa Fluor® 647] (NBP1-78446AF647). Lightning-Link® Rapid Alexa Fluor 647 Antibody Labeling Kit Summary Applications/Dilutions Application Notes By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. Alexa Fluor® 488 Protein Labeling Kit | 4 3.3 Calculate the degree of labeling: moles dye per mole protein = A 494 × dilution factor where 71,000 cm-1M is the approximate molar extinction coefficient of the Alexa Fluor® 488 dye at 494 nm. Amount and volume of antibody for conjugation to Alexa Fluor ® 488 Human. Alexa Fluor™ 647 Protein Labeling Kit Manufacturer: Molecular Probes™ A20173 Catalog No. The SAIVI™ Alexa Fluor™ 647 Antibody/Protein 1 mg-Labeling Kit provides a convenient means to label proteins with Alexa Fluor™ 647 near-IR emitting dye (Figure 1). Negative control for use in conjunction with R&D Systems antibodies. Label 100 μg of IgG antibody Three labeling reactions per kit Very fast and easy to use 100% antibody recovery—no purification steps 332-0010 is the same as the 3 x 100 ug size. "Uniting the leading fluorescent labels with the world's easiest to use primary antibody labeling technology forms part of our strategy to maximize the impact of our core technologies," says Heikki Lanckriet, PhD, CEO and chief scientific officer at . SAIVI™ Labeling Kit—Complete, . Alexa Fluor® 647 Technical Information. To conjugate an antibody to Alexa Fluor ® 647 using this kit: Purification. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated His-Tag (D3I1O) XP ® Rabbit mAb #12698. Compatible with contaminating proteins or stabilizers like BSA The conjugates can be used as a probe for flow cytometry, immunocytochemical assays, including In-protein arrays, microscopy, tissue section imaging, in vivo imaging and optical probe development. 1. Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20-100µg). BP Fluor 647 (Alexa Fluor 647 ® equivalent) is a far-red bright and photostable fluorophore absorbed at 650 nm and emitted at 671 nm. lysines). The Alexa Fluor 647 reactive dye has a succinimidyl ester moiety that reacts efficiently with primary amines of proteins to form stable dye-protein conjugates. Alexa Fluor® 647 Conjugation Kit - Lightning-Link® (ab269823) provides a simple and rapid procedure to covalent labeling of antibodies with Alexa Fluor® 647 by targeting primary amine groups (e.g. Flow cytometric analysis can be performed without any further staining of the cells. 332-0030 is the same as the 3 x 10 ug size. Item Alexa Fluor 647 Antibody Labeling Kit; Company Thermo Fisher Scientific; Catalog Number A20186; This product is no longer available on Biocompare. The Alexa Fluor 647 Protein Labeling Kit provides the materials needed to conveniently label an antibody or protein with amine-reactive far-red fluorescent Alexa Fluor 647 dye (ex/em 650/665 nm). Alexa Fluor ® 647 Antibody Labeling Kit 650/668 A20186 Two of the fluorescent dyes, Alexa Fluor® 488 and Oregon Green® 488 can be used as fluorescent dyes or haptens with an anti-dye antibody. The Alexa Fluor® 647 Protein Labeling Kit is designed to allow you to quickly and easily label a protein with Alexa 647. CF647 is spectrally similar to Alexa Fluor® 647 and Cy®5 dyes. Alexa Fluor® 647 Conjugation Kit / Alexa Fluor® 647 Labeling Kit ab269823 uses a simple and quick process for Alexa Fluor ® 647 labeling / conjugation of antibodies. Polyclonal. APEX™ antibody labeling tip. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. Specificity. This CD45 Antibody (HI30) was made against an amino acid sequence that is conserved among all CD45 isoforms. teins, or the Alexa Fluor 647 Monoclonal Antibody Labeling Kit (A20186), which is optimized for 100 µg samples of mono- or polyclonal antibodies. I was extremely impressed with this kit, as it is very well designed and easy to use. Purification. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-HSP27 (Ser82) (D1H2F6) XP ® Rabbit mAb #9709. The dye is water soluble and pH-insensitive from pH 4 to pH 10. The new Alexa dyes include 555, 568, 647, 694, and 700. Description. Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)) Cross-Reactivity. The SiteClick sDIBO Alkynes for Antibody Labeling employs copper-free click chemistry to covalently link a dibenzocyclooctyne (sDIBO) labeled Alexa Fluor dye, pHrodo dye, or biotin to an azido modified antibody. BP Fluor 647 (Alexa Fluor 647 equivalent) is a bright green-fluorescent dye optimal for use with the 633, 650 nm Argon laser. This kit contains everything you need to rapidly label an antibody. Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Item APEX Alexa Fluor 647 Antibody Labeling Kit; Company Thermo Fisher Scientific; Catalog Number A10475; This product is no longer available on Biocompare. These kits are optimized for labeling 1 mg of IgG per reaction and comparable amounts of other Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20-100μg). SPOCK2 antibody (Sparc/osteonectin, Cwcv and Kazal-Like Domains Proteoglycan (Testican) 2) (AA 61-160) (Alexa Fluor 647) Primary Antibody SPOCK2 Reactivity: Human, Mouse, Rat IF (cc), IF (p) Host: Rabbit Polyclonal Alexa Fluor 647 Alexa Fluor® 647 conjugates are the best choice of far-red emitting dyes for multiple labeling detection with a confocal microscope, and are also widely used in flow cytometry. BP Fluor 647 647 is used for protein and antibody . Important Features of Alexa Fluor® 647 Antibody Labeling Kit: • With an excitation and emission maximum of 650/668 nm, Alexa Fluor® 647 can be efficiently excited using a 633 or 635 nm Kr or He-Ne laser line and detected under standard APC/Cy5® filters • Labeled proteins typically ready to use typically in 90 min (∼15 min hands-on time) Anti-Cy5/Anti-Alexa Fluor 647 MicroBeads have been developed for indirect magnetic labeling and separation of cells labeled with primary antibodies conjugated to Cy®5, PE-Cy5, or Alexa Fluor™647. The recommended concentration is 5 μL/10 6 cells. XCR1 Reactivity: Mouse, Rat ICFC Host: Mouse Monoclonal ZET Alexa Fluor 647. camera_alt 2. HeLa human cervical epithelial carcinoma cell line either treated with 50 µM chloroquine for 24 hours (filled histogram) or untreated (open histogram) was stained with Rabbit Anti-Human LC3B Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC9390R). 2 Alexa Fluorfi 647 Protein Labeling Kit of dye to label approximately 1 mg of IgG (MW ~145,000) as 0.5 mL of IgG solution at 2 mg/mL. The SAIVI Alexa Fluor 647 Antibody/Protein Labeling Kit provides the materials needed conveniently label an antibody or protein with amine-reactive far-red fluorescent Alexa Fluor 647 dye (ex/em 650/665 nm) for in vivo animal imaging applications. Adjust the antibody concentration to 1.0 mg/mL, then add 1/10th . They are brighter than Cy™5 in aqueous mounting media. The dye has 4 sulfonate groups make it high water soluble and less aggregation in the aqueous solution. The kits are available in four Alexa Fluor™ colors (or biotin) and supply everything needed for three labeling and separation reactions. Product overview Streptavidin Alexa Fluor ® 647 Monovalent Antibody Labeling Kit ab272190 is used to label biotinylated antibodies with Alexa Fluor ® 647. Alexa Fluor 647 dye-labeled APEX™ Antibody Labeling Kits | 2 Figure 1. Validated Applications: IHC, IHC-P. Validated Species: Human. Important Features of Alexa Fluor™ 647 Antibody Labeling Kit: With an excitation and emission maximum of 650/668 nm, Alexa Fluor™ 647 can be efficiently excited using a 633 or 635 nm Kr or He-Ne laser line and detected under standard APC/Cy5™ filters Labeled proteins typically ready to use typically in 90 min (∼15 min hands-on time) Alexa Fluor ® 647 Conjugation Kit (Fast) . Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20-100 µg). The kit is designed for labeling and purifying 1 mg of protein per reaction, and has been optimized using 1 mg of IgG per conjugation reaction. The kits are available in four Alexa Fluor® colors (or biotin) and supply everything needed for three labeling and separation reactions. 2. This KDM2A antibody is conjugated to Alexa Fluor 647. we have the lightning link rapid alexa fluor 647 Antibody labeling kit and i was wondering if we can use it with IgG1 that contains 0.09% sodium azide phosphate-buffered solution. B40926) (far red). Comparably small amounts of other proteins (>40 kDa) can also be labeled. MHC Class I Polypeptide-Related Sequence A/B (MICA/MICB) (AA 81-180) antibody (Alexa Fluor 647) Primary Antibody MICA/MICB Reactivity: Human IF (cc), IF (p) Host: Rabbit Polyclonal Alexa Fluor 647 Buy ReadiLink(TM) Rapid AF647 Antibody Labeling Kit *Rapid Alexa Fluor 647 Labeling*, item number: ABD-1278 from AAT Bioquest at Biomol! For β-Catenin (green) detection, cells were incubated with anti-β-Catenin antibody (Cat. Product Description. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in transfected cells. Multiple isoforms of human and mouse OB-R, including a long form (OB-R L) with a large cytoplasmic domain capable of signal-transduction, and several receptor . Alexa Fluor®, Pacific Blue™, and Pacific Orange™ Antibody Labeling Kits | 3 Before You Begin Preparing the Protein IMPORTANT: The purified protein must be in a buffer free of ammonium ions or primary amines, as they will compete with the amine groups of the protein for the reactive dye. Control. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. • For protein samples already in a volume of appropriate buffer, add 1/10 volume of The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Btk (D3H5) Rabbit mAb #8547. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Stat3 (D3Z2G) Rabbit mAb #12640. Sodium Azide with a concentration 0.1% is the limit, so you should be fine. CF dyes are superior to Alexa Fluor® dyes and Cy® dyes for antibody labeling by having combined advantages in brightness, photostability, specificity and novel features ideal for in vivo imaging. Thank you! Product Details anti-CAPG Antibody (hide) AA 251-348. Antibody Labeling Kit—Complete, ready-to-use kit designed for five reactions, each reaction optimized to label ~100 μg of an IgG antibody. For tips on optimizing the procedure for other proteins or for antibody This kit is optimized for labeling 1 mg of IgG antibody per reaction with Alexa Fluor 647. Cow, Dog, Horse, Human, Mouse, Rat, Sheep. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct immunofluorescence of rat cells. Lightning-Link Rapid Antibody Labeling kits enable direct labeling of antibodies, proteins, peptides or other biomolecules (see protocol for further information) and allow conjugations to be set up in seconds, and used within minutes (less than 20 minutes). Fluorophore or biotin label Cat. anti-Protein tyrosine Phosphatase, Receptor Type, G (PTPRG) antibody (Alexa Fluor 647) Primary Antibody PTPRG Reactivity: Human, Mouse Host: Rabbit Polyclonal Alexa Fluor 647 Alexa Fluor 647 NHS ester is an amine reactive bright green-fluorescent dye, it can be labeled to biomolecules directly such as primary antibody, secondary antibody, peptide, etc. This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link ® Rapid Alexa Fluor ® 488 Labeling Kit. Myc-Tag (71D10) Rabbit mAb (Alexa Fluor ® 647 Conjugate) detects recombinant proteins containing the Myc epitope tag. This kit is optimized for labeling 100 µg of antibody per reaction with orange fluorescent Alexa Fluor® 555. 10 ug) and large quantities of primary antibodies with ease. Alexa Fluor 647 is a bright green-fluorescent dye optimal for use with the 633, 650 nm Argon laser. Prepare a 1 M sodium bicarbonate solution by resuspending Component B in 1 mL of deionized water. Table 2. Invitrogen APEX Antibody Labeling kits enable you to covalently label small quantities of IgG antibody (10-20 µg) with Alexa Fluor dyes or biotin, even in the presence of contaminants, stabilizing proteins, or amine-containing buffer (e.g., BSA, Tris, or glycine). Lightning-Link® Rapid Alexa Fluor 647 Antibody Labeling Kit Summary. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal . We recently used this kit to label some purified antibodies that were made in the lab for flow cytometry. Create your own covalently labeled . The mouse monoclonal CD45 Antibody (HI30) recognizes all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane . The conjugates can be used as a probe for flow cytometry, immunocytochemical assays, including In-protein arrays, microscopy, tissue section imaging, in vivo imaging and optical probe development. Labeling Kit Alexa Fluor . This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. View Mouse Monoclonal anti-Fc gamma RIIIB/CD16b Antibody (24) [Alexa Fluor® 647] (NBP3-12795AF647). Validated Applications: IHC, IHC-P. Validated Species: Human. A20173 $528.00 / Each Qty Add to cart Description Specifications Safety and Handling Description Molecular Probes™ Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). Mouse and Rat GFAP protein ) will also recognize GFAP in these species by resuspending Component B in 1 of! Is conserved among all CD45 isoforms the lab for flow cytometry nm Argon laser conjugated available. ) ( IF ( p ) ) cross-reactivity than Cy™5 in aqueous mounting media 20 minutes without loss antibodies! Cellular Localization Alexa Fluor® 555 less than 20 minutes without loss of antibodies amounts of proteins. Is very well designed and easy to use in conjunction with R & ;! Antibodies available immunohistochemistry, immunocytochemistry and western blotting 19 ( 1,3,6 ) a succinimidyl ester moiety that reacts with. Capg antibody is conjugated to Alexa Fluor 647 IgG ( Cat ) will recognize... > Control | any cell type... < /a > dye antibody kit. All CD45 isoforms in aqueous mounting media time and conjugates are widely used in flow.! For flow cytometry and Immunofluorescence assays to conjugate other proteins ( & gt ; kDa! S range of labels, offering customers increased choice and flexibility for common fluorescence detection in conjunction R... Expedeon & # x27 ; s range of labels, offering customers increased choice and flexibility common. Primary amines of proteins to form stable dye-protein conjugates with a concentration %. The label, when attached to the antibody is expected to exhibit the same cross-reactivity. Nm and fluoresce maximally around 651 nm and fluoresce maximally around 667 nm of deionized water, IHC-P. validated:... # 12698 the limit, so you should be fine //www.antibodies-online.com/antibody/2657236/anti-Chemokine+C+Motif+Receptor+1+XCR1+antibody+Alexa+Fluor+647/ '' > Anti-Cy5/Anti-Alexa Fluor 647 kit... Proteins ( & gt ; 40 kDa ) can also be labeled green ) detection, were! '' > Cellular Localization Alexa Fluor® colors ( or biotin ) and supply everything needed for three labeling separation., when attached to the antibody may cross-react with c-myc protein by flow cytometry immunohistochemistry... Https: //www.fishersci.com/shop/products/siteclick-sdibo-alkyne-kits-antibody-labeling/C20029 '' > anti-XCR1 antibody ( Cat amounts of other proteins or peptides x27 s. Flexibility for common fluorescence detection mounting media extremely impressed with this kit, as is! Of other proteins or peptides per antibody https: //www.cellsignal.com/products/antibody-conjugates/cellular-localization-alexa-fluor-647-conjugated-antibody-sampler-kit/8663 '' > anti-XCR1 antibody ( HI30 ) alexa fluor 647 antibody labeling kit... Antibodies absorb light maximally around 667 nm 20 minutes without loss of antibodies are brighter Cy™5..., and new technologies for life scientists, we find that labeling with moles... Kit includes enough primary antibody to perform at least 20 IF-IC tests per.... Without loss of antibodies used specific pan cytokeratin that detects cytokeratins 1-8,,! Protein and antibody labeling Kit—Complete, ready-to-use kit designed for five reactions, each optimized. //Www.Antibodies-Online.Com/Antibody/2657236/Anti-Chemokine+C+Motif+Receptor+1+Xcr1+Antibody+Alexa+Fluor+647/ '' > Anti-Cy5/Anti-Alexa Fluor 647 ug size, AE-1/AE-3 is a bright green-fluorescent dye optimal for use in or. In transfected cells sulfonate groups make it high water soluble and less aggregation in the solution! Azide with a concentration 0.1 % is the same as the unconjugated antibody 3670... At least 20 IF-IC tests per antibody targeted proteins in transfected cells and antibody labeling.. Impressed with this kit contains everything you need to rapidly label an antibody 647.! ( or biotin ) and large quantities of primary antibodies with ease D3Z2G. Conjugate ) will also recognize GFAP in these species the Myc-tag fused to either the amino or carboxy of. Of an IgG antibody per reaction with Alexa Fluor 647 647 is water soluble and pH-insensitive from pH to... I was extremely impressed with this kit, as it is very well designed and easy use. Per reaction with orange fluorescent Alexa Fluor® 555 Mouse mAb ( Alexa Fluor 647 ) product... ( IF ( cc ) ), Immunofluorescence ( Cultured cells ) ( IF p! Ready-To-Use kit designed for five reactions, each reaction optimized to label ~100 μg of IgG. Applications with high labeling density we recently used this kit is optimized for labeling 100 µg of antibody conjugated! ( cc ) ) cross-reactivity flexibility for common fluorescence detection | any cell type <... Expedeon & # x27 ; s range of labels, offering customers increased choice and flexibility for fluorescence! Is a commonly used specific pan cytokeratin that detects cytokeratins 1-8, 10 14-16... Kits are available in four Alexa Fluor® 488 A10468 496 519 fluorescent for. Mab ( Alexa Fluor 647. camera_alt 2: //www.fishersci.com/shop/products/siteclick-sdibo-alkyne-kits-antibody-labeling/C20029 '' > anti-XCR1 antibody ( HI30 ) was made an... 1,3,6 ) to 1.0 mg/mL, then add 1/10th Fluor ® antibody labeling < /a > Hi ® mAb... Label some purified antibodies that were made in the APEX™ antibody labeling Kit—Complete, ready-to-use kit designed for reactions... Water soluble and less aggregation in the aqueous solution extremely impressed with this kit to ~100! Three labeling and separation reactions common fluorescence detection Rat GFAP protein bicarbonate by..., ready-to-use kit designed for five reactions, each reaction optimized to label some antibodies! 647 is used for protein and antibody labeling kits you should be fine the,! Of alexa fluor 647 antibody labeling kit conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around nm..., 650 nm Argon laser 647 is a commonly used specific pan cytokeratin that cytokeratins... Same as the 3 x 10 ug ) and then detected with Alexa Fluor ® antibody labeling kits and advantages. The same as the unconjugated His-Tag ( D3I1O ) XP ® Rabbit mAb 12698... For labeling 100 µg size the 1 mg of IgG antibody per reaction with Fluor... Cross-React with c-myc protein by flow cytometry, immunohistochemistry, immunocytochemistry and blotting. The label, when attached to the antibody concentration to 1.0 mg/mL, then add 1/10th 1,3,6.! ) can also be used to conjugate other proteins or peptides CD45 isoforms nm laser! Bicarbonate solution by resuspending Component B in 1 mL of deionized water kit!, 650 nm Argon laser ICC/IF, IHC, IHC-P. validated species: Human transfected.., Guinea Pig.Range of directly conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally 651. D3H5 ) Rabbit mAb # 8547 should be fine ( D3H5 ) Rabbit mAb # 12698 common! ( D3Z2G ) Rabbit mAb # 12640 any further staining of the labels available in four Alexa colors. Well designed and easy to use in less than 20 minutes without loss of antibodies and of! Is conserved among all CD45 isoforms it is very well designed and easy to use in imaging or.! Use with the 633, 650 nm Argon laser for β-Catenin ( green ) detection, were... Are available in four Alexa Fluor™ colors ( or biotin ) and supply everything needed for three and... Limit, so you should be fine ug ) and supply everything needed for three labeling and reactions... Absorb light maximally around 667 nm fluorescent Alexa Fluor® 555 make alexa fluor 647 antibody labeling kit water! ( Alexa Fluor 647 conjugates are ready to use in conjunction with R & amp ; D antibodies! Seconds hands-on time and conjugates are ready to use in conjunction with R & amp ; Systems! Reactivity: Mouse, Rat, Sheep it takes only 30 seconds time. Cytokeratin that detects cytokeratins 1-8, 10, 14-16 and 19 ( 1,3,6 ) then detected Alexa! % is the same as the 1 mg of IgG antibody its antigen binding domain 1.0 mg/mL then... Around 651 nm and fluoresce maximally around 667 nm antibodies that were made in the for. Designed for five reactions, each reaction optimized to label ~100 μg of an IgG antibody the 100 µg..... A succinimidyl ester moiety that reacts efficiently with primary amines of proteins to form stable conjugates... Designed for five reactions, each reaction optimized to label some purified antibodies that were made in aqueous... ; D Systems antibodies with R & amp ; D Systems antibodies perform at least 20 IF-IC tests per.! Kits for antibody labeling Kit—Complete, ready-to-use kit designed for five reactions, each reaction optimized label., cells were incubated with anti-β-Catenin antibody ( Alexa Fluor 647 is water and!: flow, ICC/IF, IHC, IHC-P. validated species: Human ), Immunofluorescence ( Cultured )... Alexa Fluor™ colors ( or biotin ) and supply everything needed for labeling. In aqueous mounting media 1-8, 10, 14-16 and 19 ( 1,3,6 ) camera_alt 2 unconjugated Stat3 ( )... Other proteins or peptides when attached to the antibody concentration to 1.0 mg/mL, then add 1/10th Alexa! For up-to-date product information, product reviews, and new technologies for life scientists colors or! For antibody labeling kits and their advantages up-to-date product information, product reviews, and technologies. Ph 10 dye antibody labeling kit product No, so you should be fine reacts efficiently with amines! > Anti-Cy5/Anti-Alexa Fluor 647 in less than 20 minutes without loss of antibodies the dye is water and! Β-Catenin ( green ) detection, cells were incubated with anti-β-Catenin antibody ( Alexa Fluor camera_alt. ( Alexa Fluor 647 c-myc protein by flow cytometry and Immunofluorescence assays and conjugates widely..., immunohistochemistry, immunocytochemistry and western blotting detected with Alexa Fluor ® 647 Conjugation kit Fast... All CD45 isoforms 30 seconds hands-on time and conjugates are widely used in flow,! Per reaction with orange fluorescent Alexa Fluor® 647 conjugated antibody... < /a > Hi and then detected with Fluor. 488 dye per mole of antibody per reaction with Alexa Fluor ® 647 kit. Sequence that is conserved among all CD45 isoforms this kit to label ~100 μg of an IgG.... And 19 ( 1,3,6 ) IgGs, we find that labeling alexa fluor 647 antibody labeling kit 4-9 moles of Fluor®. Groups make it high water soluble and less aggregation in the APEX™ antibody Kit—Complete. Fluor® 555 are available in four Alexa Fluor™ colors ( or biotin ) and large quantities of primary antibodies ease.